基本信息
产品名称：Recombinant EpCAM Antibody
规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
应用：WB, IF/ICC, IP, Flow Cytometry, IHC
特异性：EpCAM
种属反应性：人(Human)
免疫原：A synthetic peptide corresponding to residues of human EPCAM. (AA 1-100)
阳性对照：WB: Hep G2, HEK293 and A431 cell lysates. IHC: Human Uterus tissue. Flow: HCT-116 cells. IP: HT-29 whole cell lysate. IF/ICC: HT-29 cells.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
克隆号：RR694
Format：Whole IgG
亚型：Rabbit IgG
轻链亚型：kappa
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：59% 10 mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.
防腐剂：0.01% Sodium azide
浓度：0.5 mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human)
克隆号：RR694
偶联：Unconjugated
图片
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Western Blot: Recombinant EpCAM Antibody (Ab101839)
Recombinant EpCAM Antibody (Ab101839) - Western Blot
All lanes: Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 39 kDa
Exposure time: 5.0 s
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IHC: Recombinant EpCAM Antibody (Ab101839)
Recombinant EpCAM Antibody (Ab101839) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling EpCAM with Recombinant EpCAM Antibody (Ab101839) at 1/3200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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Flow Cytometry: Recombinant EpCAM Antibody (Ab101839)
Recombinant EpCAM Antibody (Ab101839) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained with Recombinant EpCAM Antibody (Ab101839) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant EpCAM Antibody (Ab101839) ( 1/800 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
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IP: Recombinant EpCAM Antibody (Ab101839)
Recombinant EpCAM Antibody (Ab101839) - IP
EpCAM was immunoprecipitated from 0.4 mg of HT-29 whole cell lysate with Recombinant EpCAM Antibody (Ab101839) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant EpCAM Antibody (Ab101839) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant EpCAM Antibody (Ab101839) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 120 s
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IF: Recombinant EpCAM Antibody (Ab101839)
Recombinant EpCAM Antibody (Ab101839) - IF
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (LumiDye™ Blue 515) (Ab220504) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab101839.html
关键字： 上皮特异性抗原抗体 ; anti- EpCAM抗体 ; 上皮细胞粘附分子抗体 ; 重组EpCAM抗体
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