| 名称 | ID-8 |
| 描述 | ID-8, a DYRK inhibitor, sustains embryonic stem cell self-renewal in long-term culture. |
| 细胞实验 | Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. (Only for Reference) |
| 激酶实验 | In Vitro Enzymatic Assay for Histone Deacetylases: In vitro activities of the 11 recombinant human zinc-dependent HDAC enzymes are detected by ?uorigenic release of 7-amino-4-methylcoumarin from substrate upon deacetylase enzymatic activity. A series of dilutions of the unique HDAC6 compound, tubacin, and SAHA are prepared with 10% DMSO in HDAC assay buffer, and 5 μL of the dilution was added to a 50-μL reaction so that the ?nal concentration of DMSO is 1% in all of the reactions. The enzymatic reactions are conducted in duplicate at 37 °C for 30 min in a 50-μL mixture containing HDAC assay buffer, 5 μg BSA, an HDAC substrate, an HDAC enzyme, and a test compound. After enzymatic reactions, 50 μL of 2× HDAC developer is added to each well, and the plate is incubated at room temperature for an additional 15 min. Fluorescence intensity is measured at an excitation of 360 nm and an emission of 460 nm using a Synergy microplate reader. Negative (no enzyme, no inhibitor, a drug with no HDAC inhibition activity) and positive controls (known HDAC inhibitor SAHA) are included in the assays. IC50 is determined at the drug concentration that results in 50% reduction of HDAC activity compared with the control. |
| 体内活性 | ID-8通过Sox2-Oct3 / 4激活维持Nanog基因表达,能够可逆地维持ESC细胞的长期培养。ID-8在Wnt存在的情况下,调节Wnt /β-连环蛋白信号,增加CBP /β-连环蛋白,来维持hESC的未分化状态,并维持细胞的增殖和存活。 |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+90% Saline : < 8.33 mg/mL (27.93 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.
DMSO : 83.33 mg/mL (279.36 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 8.33 mg/mL (27.93 mM), Suspension.
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| 关键字 | tyrosine | stem | regulated | phosphorylation | kinase | Inhibitor | inhibit | ID-8 | ID 8 | ESC | embryonic | DYRK | dual-specificity | Dual specificity tyrosine regulated kinase | Dual specificity tyrosine phosphorylation regulated kinase | cell |
| 相关产品 | Harmine hydrochloride | MBM-55S | 2-Amino-4-hydroxypyrrolo[2,3- d]pyrimidi | ML 315 | EHT 1610 | CK2/ERK8-IN-1 | AnnH31 | LDN-192960 | ARN25068 | YK-2-69 | GNF4877 | RD0392 |
| 相关库 | 抑制剂库 | 经典已知活性库 | 抗癌化合物库 | 已知活性化合物库 | 细胞周期化合物库 | 激酶抑制剂库 | NO PAINS 化合物库 | 干细胞分化化合物库 | 膜蛋白靶向化合物库 | 酪氨酸激酶分子库 | 蛋白翻译后修饰化合物库 |