WNT8B抗体

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P12423(WNT8B Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The Wnt8b antibody is a crucial tool in studying the Wnt signaling pathway, which plays a pivotal role in embryonic development, tissue homeostasis, and disease. WNT8B, a member of the Wnt protein family, is a secreted glycoprotein involved in the Wnt/β-catenin signaling cascade. It regulates cell proliferation, differentiation, and polarity by binding to Frizzled receptors and co-receptors like LRP5/6. WNT8B is particularly significant in neural patterning, eye development, and skeletal morphogenesis. Dysregulation of WNT8B has been linked to developmental disorders and cancers, including neuroblastoma and endocrine tumors.

The Wnt8b antibody is designed to detect and quantify WNT8B protein expression in various experimental models, such as immunohistochemistry, Western blotting, and immunofluorescence. Researchers use it to investigate spatial-temporal expression patterns during embryogenesis, tissue regeneration, or disease progression. Commercially available antibodies are typically raised in rabbits or mice, targeting specific epitopes within the N-terminal or C-terminal regions of the WNT8B protein. Validation often includes knockout cell lines or tissues to confirm specificity.

Studies employing Wnt8b antibodies have advanced our understanding of its role in brain development, particularly in the midbrain-hindbrain boundary and retinal organization. In cancer research, it helps explore WNT8B's oncogenic potential through aberrant pathway activation. However, challenges remain, including cross-reactivity with homologous Wnt family members and variability in post-translational modifications. Proper controls and validation are essential for reliable data interpretation. This antibody remains indispensable for dissecting Wnt8B's functional mechanisms in developmental biology and disease pathology.