ZNF420抗体

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P12448(ZNF420 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The ZNF420 antibody is a research tool designed to target the zinc finger protein 420 (ZNF420), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family. ZNF420. also known as APAK (ATM- and p53-associated KZNF protein), is encoded by the ZNF420 gene in humans and plays roles in transcriptional regulation, DNA damage response, and cellular apoptosis. It contains characteristic C2H2-type zinc finger domains and a KRAB domain, which mediates protein-protein interactions, particularly with co-repressors like KAP1 to regulate gene silencing. Studies suggest ZNF420 interacts with tumor suppressor p53 and ATM kinase, modulating p53 activity under oxidative stress to prevent excessive apoptosis.

Antibodies against ZNF420 are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable detection of ZNF420’s expression and localization in tissues or cultured cells via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These tools have been critical in exploring ZNF420’s dual roles in cancer, where it may act as an oncogene or tumor suppressor depending on context. Commercial ZNF420 antibodies are often validated for specificity using knockout controls or siRNA-mediated silencing. Researchers utilize them to investigate ZNF420’s involvement in developmental processes, stress responses, and diseases, including certain leukemias and solid tumors. Proper validation remains essential due to potential cross-reactivity with other zinc finger proteins.