Calcein Red-AM(Biolegend 425205) 新品

维百奥生物库存现货，特价销售Calcein Red-AM！

| 产品信息

品名：Calcein Red-AM

别名：Calcein, Calcien Red-AM

品牌：Biolegend

货号：425205

规格：10 x 25 µg

| 产品描述

Calcein Red-AM is a fluorogenic, cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases. When the acetoxymethyl ester is intact the probe is non-fluorescent, but when it is cleaved by nonspecific esterases present in healthy cells, the probe can be excited and emit at 560 nm/574 nm respectively. The signal of Calcein Red-AM is proportional to the cell vitality, as esterase activity decreases in cells with poor vitality. This cell-permeant probe is not retained with fixation and permeabilization.

| 产品细节

1）Preparation

Calcein Red-AM labeling kit consists of lyophilized Calcein Red-AM and anhydrous DMSO. For reconstitution, bring the kit to room temperature and add 25 µL of DMSO to one vial of Calcein Red-AM dye until fully dissolved.

2）Storage & Handling

Calcein Red-AM should be stored at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Calcein Red-AM, use immediately or store at -20°C in a dry place and protected from light, preferably in a desiccator or in a container with desiccant for no more than a month.

3）Application

FC - Quality tested； Live cell imaging - Verified

4）Recommended Usage

This lot has been tested by flow cytometric analysis of cell vitality. It can be used at concentrations ranging from 0.025 – 0.006 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.

5）Application Notes

The molecular weight of Calcein Red-AM is 1015.79 Da. The maximum excitation and emission wavelengths of Calcein Red-AM are 560 nm/574 nm, respectively. Each 25 µg of Calcein-AM may be reconstituted with 25 µL of anhydrous DMSO to yield a stock concentration of 1 mM.