The HSD17B4 antibody is a crucial tool for studying the 17β-hydroxysteroid dehydrogenase type 4 (HSD17B4) enzyme, a multifunctional protein encoded by the HSD17B4 gene in humans. This enzyme, also known as peroxisomal D-bifunctional protein (DBP), plays a key role in lipid metabolism, particularly in the β-oxidation of very-long-chain fatty acids and bile acid synthesis within peroxisomes. It belongs to the short-chain dehydrogenase/reductase (SDR) superfamily and exhibits dual enzymatic activities: 17β-hydroxysteroid dehydrogenase and 3-hydroxyacyl-CoA dehydrogenase. Dysregulation of HSD17B4 is linked to peroxisomal disorders like D-bifunctional protein deficiency (a subtype of Zellweger spectrum disorders) and has been implicated in hormone-related cancers due to its role in steroid metabolism. Researchers employ HSD17B4 antibodies in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression patterns, subcellular localization, and involvement in metabolic pathways or disease mechanisms. Commercial HSD17B4 antibodies are typically raised in rabbit or mouse hosts, with validation in specific applications and species. Its study contributes to understanding lipid homeostasis, steroid hormone regulation, and peroxisome-related pathologies.