The NDUFA5 antibody is a crucial tool for studying mitochondrial complex I (NADH:ubiquinone oxidoreductase), a key component of the electron transport chain. NDUFA5 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5) is a nuclear-encoded subunit of complex I, which plays a vital role in ATP synthesis by facilitating electron transfer from NADH to ubiquinone. Dysregulation of NDUFA5 has been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s), and certain cancers.
NDUFA5 antibodies are widely used in research to detect protein expression levels via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help investigate mitochondrial dysfunction mechanisms, assess complex I assembly defects, and explore metabolic adaptations in diseases. Species specificity typically covers human, mouse, and rat samples, with common hosts including rabbit (polyclonal) and mouse (monoclonal).
Validation of NDUFA5 antibodies often involves knockout cell lines or tissue controls to confirm specificity. Recent studies also utilize these antibodies to probe NDUFA5’s role in oxidative stress responses and its potential as a biomarker for metabolic syndromes. Researchers must optimize experimental conditions, as mitochondrial protein extraction and antibody dilution ratios can significantly impact results.