The RNF115 antibody is a research tool designed to detect and study the Ring Finger Protein 115 (RNF115), also known as BCA2 (Breast Cancer-Associated Gene 2), an E3 ubiquitin ligase belonging to the RING finger protein family. RNF115 plays a critical role in ubiquitination, a post-translational modification that regulates protein degradation, localization, and interactions via the ubiquitin-proteasome system. It is implicated in diverse cellular processes, including viral infection response, cancer progression, and metabolic regulation. For instance, RNF115 interacts with viral proteins to modulate immune responses and has dual roles in cancer—acting as a tumor suppressor in breast cancer by promoting EGFR degradation, yet potentially driving metastasis in other contexts.
RNF115 antibodies are typically generated in hosts like rabbits or mice using immunogenic peptide sequences or recombinant protein fragments. These antibodies enable techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate RNF115 expression, localization, and interactions in cell lines, tissues, or disease models. Commercial RNF115 antibodies vary in clonality, epitope specificity, and validation data, requiring careful selection for experimental reliability. Research utilizing these antibodies has advanced understanding of RNF115's roles in diseases like hepatitis C, breast cancer, and obesity, highlighting its potential as a therapeutic target. Validation via knockout controls or siRNA remains essential to confirm antibody specificity in experimental settings.