The RPS21 antibody is a valuable tool for investigating the function and expression of ribosomal protein S21 (RPS21), a component of the 40S ribosomal subunit. RPS21 plays a critical role in mRNA translation, ribosome biogenesis, and has been implicated in extraribosomal functions, including cell cycle regulation, apoptosis, and DNA repair. Studies suggest its dysregulation may contribute to cancer progression, viral infection responses, and neurodegenerative disorders.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect RPS21 expression levels, subcellular localization, and interactions in various biological samples. It is typically produced in hosts such as rabbits or mice, targeting specific epitopes of human RPS21. with cross-reactivity often validated in model organisms like mice and rats.
Quality control includes validation via siRNA knockdown, peptide blocking assays, or recombinant protein recognition to ensure specificity. Researchers utilize the RPS21 antibody to explore its role in diseases; for example, elevated RPS21 levels correlate with poor prognosis in cancers like hepatocellular carcinoma, while reduced expression may link to neurodegenerative mechanisms. Its application aids in elucidating ribosome-related pathologies and potential therapeutic targets.