BCO2抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09356(BCO2 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P09356(BCO2 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

**Background of BCO2 Antibody**

BCO2 (β-carotene oxygenase 2), also known as BCDO2. is a mitochondrial enzyme involved in the cleavage of carotenoids, particularly β-carotene, into apocarotenoids and vitamin A derivatives. Unlike its paralog BCO1. which localizes to the cytosol and preferentially cleaves provitamin A carotenoids, BCO2 exhibits broader substrate specificity, targeting non-provitamin A carotenoids like lutein and zeaxanthin. This enzyme plays a critical role in carotenoid homeostasis, influencing tissue-specific accumulation and protecting against carotenoid-induced mitochondrial dysfunction.

BCO2 antibodies are essential tools for studying its expression, localization, and function in metabolic pathways. They enable detection of BCO2 protein levels in tissues such as the liver, retina, and adrenal glands, where carotenoid metabolism is active. Research using these antibodies has linked BCO2 activity to physiological processes, including vision, antioxidant defense, and lipid metabolism. Dysregulation of BCO2 is implicated in conditions like age-related macular degeneration and metabolic disorders.

Antibodies targeting BCO2 are typically developed using recombinant protein fragments or peptide sequences unique to the enzyme. They are validated via Western blot, immunohistochemistry, and immunofluorescence, often cross-reacting across species (e.g., human, mouse, rat). Challenges include ensuring specificity due to structural similarities with BCO1 and accounting for tissue-specific isoforms. Recent studies also highlight BCO2's role in mediating interactions between diet, genetics, and disease, underscoring the antibody's utility in nutrigenomics and translational research.