The RNA Pseudouridine Synthase Domain-Containing 2 (RPUSD2) antibody is a tool used to study the RPUSD2 protein, a member of the pseudouridine synthase family involved in RNA modification. Pseudouridylation, a common post-transcriptional RNA modification, regulates RNA stability, translation, and cellular stress responses. RPUSD2 is implicated in catalyzing this process, particularly in ribosomal RNA (rRNA) processing, though its exact substrates and mechanisms remain under investigation.
RPUSD2 antibodies are primarily employed in detecting protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Research suggests RPUSD2 localizes to the nucleolus, hinting at roles in ribosome biogenesis. Dysregulation of pseudouridine synthases, including RPUSD2. has been linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. For instance, altered RPUSD2 expression has been observed in hepatocellular carcinoma and breast cancer, potentially influencing tumor progression through ribosome dysfunction or impaired RNA metabolism.
Despite its emerging significance, RPUSD2's functional landscape is not fully mapped. Current studies focus on its interplay with other RNA-modifying enzymes, stress adaptation pathways, and disease-specific contexts. The RPUSD2 antibody thus serves as a critical reagent for elucidating its biological roles and therapeutic potential in RNA-mediated pathologies.