Rat IgG2a-HRP is a secondary antibody conjugated to horseradish peroxidase (HRP), commonly used in immunoassays to detect primary antibodies of the rat IgG2a subclass. Rat IgG2a, an immunoglobulin subclass, plays a role in adaptive immune responses, particularly in opsonization and complement activation. The HRP conjugation enables enzymatic signal amplification in detection methods like Western blotting, ELISA, or immunohistochemistry.
This antibody is typically produced in a host species (e.g., mouse, goat, or rabbit) immunized with rat IgG2a. It specifically binds to the Fc region of rat IgG2a antibodies, allowing researchers to visualize antigen-antibody interactions when paired with chromogenic or chemiluminescent substrates. Its high specificity minimizes cross-reactivity with other rat IgG subclasses (e.g., IgG1. IgG2b, IgG2c) or immunoglobulins from other species, ensuring reliable results.
Applications include quantifying immune responses, tracking protein expression, and validating disease biomarkers. Proper use requires optimization of dilution ratios and blocking steps to reduce background noise. Stored at 4°C for short-term or -20°C for long-term stability, it remains a critical tool in biomedical research for its sensitivity, versatility, and compatibility with high-throughput workflows.