NDUFA4抗体;NDUFA4 Antibody—艾普蒂

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-4: SKOV3 cells, human fetal liver tissue, human fetal brain tissue, Hela cells, Primary antibody: P11969(NDUFA4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P11969(NDUFA4 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P11969(NDUFA4 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

NDUFA4 (NADH:ubiquinone oxidoreductase subunit A4) is a critical component of mitochondrial Complex IV (cytochrome c oxidase), the terminal enzyme in the mitochondrial electron transport chain (ETC) responsible for transferring electrons to oxygen and maintaining cellular energy production. Initially misclassified as a subunit of Complex I due to its nomenclature, NDUFA4 was later confirmed to structurally and functionally associate with Complex IV, playing a role in its stability and activity.

Antibodies targeting NDUFA4 are essential tools for studying mitochondrial function, ETC dynamics, and oxidative phosphorylation (OXPHOS) defects. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and mitochondrial integrity in various disease models. Dysregulation of NDUFA4 has been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Alzheimer’s), and cancer, where altered energy metabolism is a hallmark. Researchers also utilize NDUFA4 antibodies to investigate cellular responses to hypoxia, metabolic stress, or mitochondrial toxins. Validated antibodies ensure specificity for distinguishing between mitochondrial and non-specific signals, aiding in the identification of biomarkers or therapeutic targets for diseases associated with mitochondrial dysfunction.