The WRNIP1 (Werner helicase-interacting protein 1) antibody is a research tool used to study the function and expression of the WRNIP1 protein, which plays a role in maintaining genome stability and DNA replication processes. WRNIP1 interacts with Werner syndrome ATP-dependent helicase (WRN), a protein critical for DNA repair, replication, and telomere maintenance. Mutations in WRN are linked to Werner syndrome, a premature aging disorder. WRNIP1 is believed to assist WRN in resolving replication stress by stabilizing stalled replication forks and facilitating the restart of DNA synthesis, though its precise mechanisms remain under investigation.
Antibodies targeting WRNIP1 are primarily utilized in molecular biology and cancer research to detect protein expression levels, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Studies suggest WRNIP1 overexpression in certain cancers may correlate with tumor progression or chemoresistance, highlighting its potential as a therapeutic target. Conversely, reduced WRNIP1 levels have been implicated in genomic instability, contributing to aging-related pathologies. Researchers also employ WRNIP1 antibodies to explore its interplay with other replication-associated proteins, such as PCNA and DNA polymerases, to elucidate its role in replication fork dynamics. Despite progress, further studies are needed to fully characterize its regulatory pathways and clinical relevance.