The RCOR2 antibody is a valuable tool for studying the REST corepressor 2 (RCOR2) protein, a member of the CoREST family. RCOR2. also known as CoREST2. functions as a transcriptional coregulator by interacting with REST (RE1-silencing transcription factor) and other chromatin-modifying complexes. It plays a critical role in repressing neuronal gene expression in non-neuronal cells and stem cells, contributing to cellular differentiation and development. RCOR2 forms complexes with histone demethylases (e.g., LSD1/KDM1A) and histone deacetylases (e.g., HDAC1/2), enabling chromatin remodeling and epigenetic regulation. Its involvement in neurogenesis, stem cell maintenance, and cancer pathways has made it a focus in developmental biology and oncology research.
The RCOR2 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and DNA-binding activity. Researchers employ it to explore RCOR2's role in diseases, particularly cancers where dysregulated RCOR2 expression correlates with tumor progression or metastasis. Specificity validation via knockout/knockdown controls is essential, as cross-reactivity with other CoREST family members (e.g., RCOR1) may occur. Commercial RCOR2 antibodies are typically raised against conserved epitopes, such as the N-terminal or C-terminal regions, with host species (rabbit, mouse) and clonality (monoclonal/polyclonal) options available. Recent studies also highlight its potential as a therapeutic target in neurological disorders and malignancies.