DACT1 (Dapper homolog 1), also known as Dact1 or Dpr1. is a cytosolic scaffolding protein belonging to the Dapper (DACT) family. It plays a regulatory role in multiple signaling pathways, including Wnt/β-catenin and TGF-β, by interacting with key components like Dishevelled (Dvl) and Smad proteins. DACT1 is involved in embryonic development, tissue morphogenesis, and cell polarity, with critical functions in neural tube closure, heart development, and limb formation. Dysregulation of DACT1 has been linked to cancers (acting as a tumor suppressor or promoter in context-dependent manners) and developmental disorders.
DACT1 antibodies are essential tools for detecting and studying the expression, localization, and function of DACT1 in research. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). They help elucidate DACT1's role in signaling crosstalk, disease mechanisms, and developmental processes. Commercially available DACT1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein's conserved regions. Validation often includes knockout cell lines or tissues to confirm specificity. Researchers rely on these antibodies to explore DACT1's dual roles in tumorigenesis, its interaction networks, and potential therapeutic implications in developmental anomalies or cancer progression.