The FBXO9 antibody is a research tool designed to detect and study the F-box protein 9 (FBXO9), a member of the F-box protein family. These proteins are critical components of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complexes, which mediate substrate recognition in the ubiquitin-proteasome system, regulating protein degradation and cellular processes such as cell cycle progression, signal transduction, and stress responses. FBXO9 specifically functions as a substrate-recognition subunit, targeting proteins for ubiquitination and subsequent proteasomal degradation. Dysregulation of FBXO9 has been implicated in various diseases, including cancer, neurodegenerative disorders, and immune dysregulation, highlighting its role in maintaining protein homeostasis and cellular health.
Antibodies against FBXO9 are widely used in molecular and cellular biology to investigate its expression, localization, and interaction partners. They enable techniques like Western blotting, immunoprecipitation, and immunofluorescence to analyze FBXO9 levels in different tissues or under experimental conditions. The specificity and sensitivity of these antibodies are rigorously validated using controls such as knockout cell lines or siRNA-mediated knockdown to ensure accurate detection. Researchers also employ FBXO9 antibodies to explore its involvement in disease mechanisms, potential therapeutic targets, and biomarker discovery. As FBXO9's substrates and regulatory pathways remain incompletely characterized, these antibodies are vital tools for advancing understanding of its biological and pathological functions.