The p60CAF1 antibody targets the p60 subunit of the Chromatin Assembly Factor 1 (CAF1) complex, a conserved histone chaperone critical for chromatin organization and DNA replication. The CAF1 complex, composed of p150. p60 (also called CHAF1B), and p48 subunits, facilitates the deposition of histone H3/H4 tetramers onto newly synthesized DNA during replication and repair, ensuring proper nucleosome assembly. The p60 subunit acts as a central scaffold, mediating interactions between the p150 and p48 subunits and stabilizing the complex. It also binds histones H3 and H4. playing a direct role in histone chaperone activity. Dysregulation of CAF1 is linked to replication stress, genomic instability, and diseases like cancer, where overexpression of p60 has been observed in certain tumors, correlating with aggressive phenotypes.
The p60CAF1 antibody is widely used in research to study chromatin dynamics, DNA replication-coupled nucleosome assembly, and epigenetic regulation. It enables detection of CAF1 complex localization via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding investigations into replication fork progression, DNA repair mechanisms, and stem cell differentiation. Commercial p60CAF1 antibodies are typically raised in rabbits or mice, validated for specificity across human, mouse, and rat samples. Its application extends to cancer biology, where it helps assess CAF1's role in tumor proliferation and metastasis, offering insights into potential therapeutic targets for precision oncology.