CytochromeP45017A1抗体,Rabbit Monoclonal CytochromeP45017A1 Antibody
  • CytochromeP45017A1抗体,Rabbit Monoclonal CytochromeP45017A1 Antibody
  • CytochromeP45017A1抗体,Rabbit Monoclonal CytochromeP45017A1 Antibody
  • CytochromeP45017A1抗体,Rabbit Monoclonal CytochromeP45017A1 Antibody

CytochromeP45017A1抗体;CytochromeP45017A1 Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-30
QQ交谈 微信洽谈

产品详情

中文名称:CytochromeP45017A1抗体英文名称:Rabbit Monoclonal CytochromeP45017A1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8569 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: CytochromeP45017A1
2025-05-30 CytochromeP45017A1抗体 Rabbit Monoclonal CytochromeP45017A1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8569 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/1000-1/2000 Human,Mouse,Rat
IF1/20-1/50 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM1/20-1/100 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesCPT7; CYP17; P450C17; S17AH; CYP17A1;;Cytochrome P450 17A1
WB Predicted band sizeCalculated MW: 57 kDa ; Observed MW: 50 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Cytochrome P450 17A1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       Western blot analysis of Cytochrome P450 17A1 expression in Jurkat cell lysate.    


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       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


  •  

     

       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


  •  

     

       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


           

参考文献

以下是关于Cytochrome P450 17A1(CYP17A1)抗体的参考文献示例,包含文献名称、作者及简要摘要内容:

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1. **文献名称**:*Molecular cloning and expression of human cytochrome P450 17A1: Immunochemical characterization and tissue distribution*

**作者**:Chung BC, et al.

**摘要**:该研究通过分子克隆技术鉴定了人CYP17A1的基因序列,并制备了特异性抗体。通过免疫印迹(Western blot)和免疫组化验证抗体在肾上腺、性腺等组织中的表达,证实了抗体的特异性与敏感性。

2. **文献名称**:*CYP17A1 expression in prostate cancer: Immunohistochemical analysis and clinical correlation*

**作者**:Yamamoto Y, et al.

**摘要**:研究利用CYP17A1特异性抗体对前列腺癌组织进行免疫组化分析,发现其表达水平与肿瘤侵袭性相关,提示CYP17A1可能作为潜在治疗靶点。抗体验证包括阴性对照和竞争性抑制实验。

3. **文献名称**:*Role of cytochrome P450 17A1 in steroidogenesis: Insights from antibody-mediated inhibition studies*

**作者**:Pandey AV, et al.

**摘要**:通过抗CYP17A1抗体阻断酶活性,探究其在类固醇激素合成中的功能。研究采用免疫沉淀和酶活性检测,证实抗体可有效抑制17α-羟化酶和17.20-裂解酶活性,为疾病模型研究提供工具。

4. **文献名称**:*Tissue-specific regulation of CYP17A1: Evidence from genetic and antibody-based profiling*

**作者**:Sherva R, et al.

**摘要**:结合基因多态性数据与抗体检测的蛋白表达(Western blot和免疫荧光),揭示了CYP17A1在不同组织中的调控差异,强调了抗体在解析酶表达模式中的关键作用。

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这些文献涵盖了抗体的开发、验证及在疾病机制研究中的应用,适用于实验设计或论文写作中的方法学参考。

       

背景信息

Cytochrome P450 17A1 (CYP17A1) is a critical enzyme in steroidogenesis, primarily expressed in the adrenal glands and gonads. It catalyzes two key reactions: the 17α-hydroxylation of pregnenolone/progesterone to form precursors for glucocorticoids and the 17.20-lyase activity required for androgen synthesis. Dysregulation of CYP17A1 is linked to endocrine disorders, such as congenital adrenal hyperplasia, polycystic ovary syndrome (PCOS), and hormone-sensitive cancers like prostate cancer. Antibodies targeting CYP17A1 are essential tools for studying its expression, localization, and function in both normal physiology and disease contexts.

CYP17A1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein levels in tissues, cell lysates, or biological fluids. These antibodies help researchers investigate tissue-specific steroidogenic activity, evaluate therapeutic responses to CYP17A1 inhibitors (e.g., abiraterone in prostate cancer), or explore genetic mutations affecting enzyme activity. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple epitopes, enhancing sensitivity. Validation via knockout controls or enzymatic assays is crucial to confirm antibody reliability.

Clinically, CYP17A1 antibodies may aid in diagnosing adrenal or gonadal tumors with aberrant steroid production. Their role in elucidating molecular pathways also supports drug development targeting steroid hormone-dependent conditions.

       
关键字: CytochromeP45017A1抗体;CytochromeP45017A1;CytochromeP45017A1 A;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
询盘

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