Phospho-KAP1(S824)抗体;Phospho-KAP1(S824) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-KAP1 (S824) expression in HeLa cell treated with etoposide.    

  
  

**Phospho-KAP1(S824) Antibody Background**

KAP1 (KRAB-associated protein 1), also known as TRIM28. is a transcriptional co-repressor involved in chromatin remodeling, DNA damage response, and epigenetic regulation. Phosphorylation of KAP1 at serine 824 (S824) is a critical post-translational modification triggered by DNA double-strand breaks (DSBs). This phosphorylation event is primarily mediated by the ATM/ATR kinases, which are activated during the DNA damage response (DDR).

The Phospho-KAP1(S824) antibody specifically detects KAP1 when phosphorylated at S824. serving as a marker for ongoing DDR signaling. This modification facilitates chromatin relaxation by promoting the dissociation of KAP1 from chromatin, enabling access for DNA repair machinery such as homologous recombination (HR) proteins. It plays a pivotal role in maintaining genomic stability and regulating cell cycle checkpoints.

Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study DDR activation, chromatin dynamics, and repair pathway efficiency in contexts like cancer, radiation exposure, or genotoxic drug treatments. Its specificity is validated via knockout controls or phosphatase-treated samples. Dysregulation of KAP1 phosphorylation is linked to genomic instability, cancer progression, and resistance to therapies, making this antibody a valuable tool in oncology and molecular biology research.