The RBMXL2 antibody is a tool used to detect RBMXL2 (RNA-Binding Motif Protein X-Like 2), a protein belonging to the heterogeneous nuclear ribonucleoprotein (hnRNP) family. RBMXL2 shares homology with RBMX, a ubiquitously expressed RNA-binding protein involved in mRNA splicing, stability, and translation. However, RBMXL2 is specifically expressed in germ cells and is critical during gametogenesis, particularly in spermatogenesis. It compensates for the loss of RBMX in meiotic and post-meiotic male germ cells, regulating the splicing and expression of genes essential for germ cell development and function. Studies suggest RBMXL2 plays a role in maintaining chromosomal synapsis and meiotic progression. The antibody is typically produced in hosts like rabbits or mice, using immunogens derived from conserved regions of RBMXL2. It is validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence to study RBMXL2's expression, localization, and interactions in reproductive tissues. Research using this antibody has advanced understanding of fertility-related mechanisms, RNA metabolism in germ cells, and disorders linked to gametogenesis defects. Its specificity is confirmed through knockout controls or siRNA-mediated knockdown, ensuring reliable detection in model organisms like mice and humans.