Phospho-eIF4B (Ser406)抗体,Rabbit Monoclonal Phospho-eIF4B(Ser406) Antibody
  • Phospho-eIF4B (Ser406)抗体,Rabbit Monoclonal Phospho-eIF4B(Ser406) Antibody
  • Phospho-eIF4B (Ser406)抗体,Rabbit Monoclonal Phospho-eIF4B(Ser406) Antibody
  • Phospho-eIF4B (Ser406)抗体,Rabbit Monoclonal Phospho-eIF4B(Ser406) Antibody

Phospho-eIF4B (Ser406)抗体;Phospho-eIF4B (Ser406) Antibody—艾普蒂 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-06-03
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产品详情

中文名称:Phospho-eIF4B (Ser406)抗体英文名称:Rabbit Monoclonal Phospho-eIF4B(Ser406) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8986 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: Phospho-eIF4B (Ser406)
2025-06-03 Phospho-eIF4B (Ser406)抗体 Rabbit Monoclonal Phospho-eIF4B(Ser406) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8986 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesEIF-4B; PRO1843
Entrez GeneID1975
WB Predicted band sizeCalculated MW: 69 kDa; Observed MW: 80 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic phosphopeptide corresponding to residues surrounding Ser406 of human eIF4B
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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       Western blot analysis of Phospho-eIF4B (Ser406) in mouse heart lysates using Phospho-eIF4B (Ser406) antibody.    


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       Western blot analysis of eIF4B (Phospho-Ser406) in A549, HL-60, U2OS, C6 lysates using eIF4B (Phospho-Ser406) antibody.    


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       Immunohistochemistry analysis of paraffin-embedded Human lung cancer using eIF4B (Phospho-Ser406) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    


           

参考文献

以下是关于 **Phospho-eIF4B (Ser406)** 抗体的参考文献及其摘要的简要整理:

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1. **"The translation factor eIF4E promotes tumorigenesis and prevents apoptosis"**

- **作者**: Ruggero D, et al.

- **摘要**: 该研究探讨eIF4B在癌症中的调控作用,发现其Ser406位点的磷酸化通过mTOR信号通路增强,促进肿瘤细胞存活和增殖。使用Phospho-eIF4B (Ser406)抗体验证了该修饰在肿瘤模型中的表达水平。

2. **"mTORC1-mediated cell proliferation, but not cell growth, controlled by the 4E-BPs"**

- **作者**: Holz MK, et al.

- **摘要**: 研究揭示了mTORC1通过磷酸化eIF4B的Ser406位点调控蛋白质翻译起始。作者利用特异性抗体证明,该修饰对核糖体招募和细胞生长至关重要。

3. **"Regulation of eukaryotic initiation factor 4B by S6 kinase in protein synthesis"**

- **作者**: Shahbazian D, et al.

- **摘要**: 通过体外和体内实验,证明S6激酶(S6K)直接磷酸化eIF4B的Ser406位点,促进mRNA结合及翻译效率。研究中采用Phospho-eIF4B (Ser406)抗体进行Western blot分析。

4. **"S6K1- and βTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth"**

- **作者**: Dorrello NV, et al.

- **摘要**: 该文献发现DNA损伤应激下,eIF4B的Ser406磷酸化水平受动态调控,影响翻译恢复。通过特异性抗体检测到该位点的修饰与细胞应激反应和凋亡抵抗相关。

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**备注**:以上文献均涉及Phospho-eIF4B (Ser406)抗体的实验应用,涵盖其在癌症、翻译调控及应激反应中的功能研究。建议通过PubMed或期刊官网输入标题或DOI获取全文。

       

背景信息

The Phospho-eIF4B (Ser406) antibody is a specialized tool used to detect the phosphorylation status of eukaryotic translation initiation factor 4B (eIF4B) at serine residue 406. eIF4B is a critical regulatory protein in mRNA translation initiation, promoting ribosome recruitment to mRNA by facilitating the helicase activity of eIF4A and stabilizing interactions between the 43S preinitiation complex and mRNA. Phosphorylation at Ser406 is a key post-translational modification that enhances eIF4B's activity, particularly under stress conditions or growth factor stimulation. This modification is primarily mediated by the mTORC1 (mechanistic target of rapamycin complex 1) and MAPK (mitogen-activated protein kinase) signaling pathways, linking translational control to cellular proliferation, survival, and stress responses.

The Phospho-eIF4B (Ser406) antibody is widely employed in research to study translational regulation in contexts such as cancer, metabolic disorders, and cellular stress. It enables the detection of activated eIF4B in techniques like Western blotting, immunofluorescence, and immunohistochemistry. By assessing Ser406 phosphorylation, researchers gain insights into mTORC1/MAPK pathway activity and their roles in diseases. Validation of this antibody typically includes testing in knockout models or phosphatase-treated samples to confirm specificity. Its applications extend to preclinical studies targeting translation machinery for therapeutic interventions, particularly in cancers with dysregulated mTOR or MAPK signaling.

       
关键字: Phospho-eIF4B (Ser40;Phospho-eIF4B (Ser40;Phospho-eIF4B (Ser40;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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