**Background of Phospho-JNK1 (Thr183/Tyr185) Antibody**
The Phospho-JNK1 (Thr183/Tyr185) antibody is a critical tool for studying the activation status of c-Jun N-terminal kinase 1 (JNK1), a member of the mitogen-activated protein kinase (MAPK) family. JNK1 plays a central role in cellular stress responses, apoptosis, proliferation, and differentiation. Its activity is tightly regulated by dual phosphorylation at conserved threonine (Thr183) and tyrosine (Tyr185) residues within the activation loop. This phosphorylation, mediated by upstream MAPK kinases (MKK4/7), induces conformational changes that enable JNK1 to phosphorylate downstream targets, including transcription factors like c-Jun.
The Phospho-JNK1 (Thr183/Tyr185) antibody specifically recognizes this phosphorylated, active form of JNK1. making it invaluable for investigating stress-activated pathways under conditions such as UV radiation, oxidative stress, cytokine exposure, or metabolic imbalance. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess JNK1 activation in diseases like cancer, neurodegenerative disorders, and inflammatory conditions.
Its specificity helps distinguish activated JNK1 from inactive forms or other JNK isoforms (JNK2/3), though cross-reactivity should be validated experimentally. This antibody is widely applied in drug discovery, mechanistic studies, and biomarker research, linking JNK1 signaling to pathophysiology and therapeutic targeting.