产品概述
| 产品名称(Product Name) | eNOS(Mix)Mouse Monoclonal Antibody |
| 描述(Description) | Mouse Monoclonal Antibody |
| 宿主(Host) | Mouse |
| 应用(Application) | WB |
| 种属反应性(Reactivity) | Human,Mouse,Rat,Rabbit |
产品性能
| 偶联物(Conjugation) | Unconjugated |
| 修饰(Modification) | Unmodified |
| 同种型(Isotype) | IgG |
| 克隆(Clonality) | Monoclonal |
| 形式(Form) | Liquid |
| 存放说明(Storage) | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 储存溶液(Buffer) | PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol. |
| 纯化方式(Purification) | Affinity purification |
免疫原
| 基因名(Gene Name) | NOS3 |
| 别名(Alternative Names) | NOS3; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII |
| 基因ID(Gene ID) | 4846 |
| 蛋白ID(SwissProt ID) | P29474 |
产品应用
| 稀释比(Dilution Ratio) | WB 1:500-2000 |
| 蛋白分子量(Molecular Weight) | 130-140kDa |
研究背景
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subcellular location:Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.,subunit:Homodimer. Interacts with NOSIP and NOSTRIN.,tissue specificity:Platelets, placenta, liver and kidney.,
研究领域
Arginine and proline metabolism;Calcium;VEGF;
关键字: NOS3;eNOS(Mix)Mouse;Monoclonal;Antibody;一抗
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