Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
分子别名（Synonym）
CD3E & CD3D,CD3 delta & CD3 epsilon
表达区间及表达系统（Source）
Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-M5219) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
蛋白结构（Molecular Characterization）
Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.1 kDa (CD3E) and 14.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 18-19 kDa and 25-32 kDa under reducing (R) condition, and 38-47 kDa under non-reducing (NR) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
T细胞表面糖蛋白CD3δ链与CD3ε链（分别简称CD3D、CD3E）均属于单次跨膜I型膜蛋白。CD3D与CD3ε链（CD3E）、CD3γ链、CD3ζ链以及T细胞受体α/β和γ/δ异源二聚体共同组装成T细胞受体-CD3复合物。该复合物在将抗原识别与细胞内多种信号转导通路相耦合的过程中发挥关键作用。
关键字： CD3E & CD3D;CD3E蛋白;CD3重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。