Biotinylated Human Fc gamma RIIIB / CD16b (NA1) Protein, His,Avitag™ (SPR & BLI & MALS verified)
分子别名（Synonym）
Fc gamma RIIIB , CD16b (NA1), FCGR3B, CD16B, FCG3B, FCGR3, FCG3, IGFR3
表达区间及表达系统（Source）
Biotinylated Human CD16b (NA1), His,Avitag (CDB-H82E4) is expressed from human HEK293 cells. It contains AA Gly 17 - Ser 200 (Accession # AAA35881.1). The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.
Predicted N-terminus: Gly 17
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 24.6 kDa. The protein migrates as 38-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
背景介绍
CD16是一种低亲和力Fc受体，包括FcγRIIIa（CD16a）和FcγRIIIb（CD16b）两种类型。这些受体可与IgG抗体的Fc段结合。CD16由两个高度同源的基因以细胞类型特异性方式编码，表达于自然杀伤细胞、中性粒细胞、单核细胞和巨噬细胞表面。
CD16B（亦称为FCGR3B、FCG3B）特异性表达于多形核白细胞（中性粒细胞）及受刺激的嗜酸性粒细胞。该蛋白是免疫球蛋白γ段Fc区的低亲和力受体，既能结合复合型/聚合型IgG，也能结合单体IgG。与III-A型受体不同，FCG3B不具备介导抗体依赖性细胞毒作用和吞噬作用的能力，其功能可能是在外周循环中捕获免疫复合物而不激活中性粒细胞。
关键字： FCG3B/CD16b;FCG3B蛋白;CD16b重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。