Human Integrin alpha E beta 7 (ITGAE&ITGB7) Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子别名（Synonym）
Integrin alpha E beta 7, ITGAE&ITGB7
表达区间及表达系统（Source）
Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7) (Accession # P38570-1 (ITGAE) & P26010-1 (ITGB7)).
Predicted N-terminus: Phe 19 (ITGAE) & Glu 20 (ITGB7)
蛋白结构（Molecular Characterization）
Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAE and ITGB7, has a calculated MW of 128.6 kDa (ITGAE) and 81.8 kDa (ITGB7). Subunit ITGAE is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 80-90 kDa and 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
整合素αEβ7由两个主要亚基组成：整合素αE（ITGAE）亦称CD103（分化簇103），而整合素β7是一种由ITGB7基因编码的蛋白质。这种整合素主要表达于黏膜组织内的T淋巴细胞系，与其他整合素相比具有显著狭窄的表达模式。表达αEβ7的淋巴细胞在肠道中含量丰富，构成人体T细胞总量的重要组成部分。
肺移植的有效性因相对较高的排斥发生率而受损。正常肺组织中存在大量与气道上皮接触的淋巴细胞，其中部分细胞表达黏膜整合素αE（CD103）β7。该整合素并非归巢受体，其主要功能被认为是将淋巴细胞滞留于上皮表面。
关键字： 整合素αEβ7;Integrin αEβ7蛋白;αEβ7重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。