 SEC-HPLC | SEC-HPLC detection for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1). |
 Bioactivity | Detects B2M/Beta-2-microglobulin in indirect ELISAs. |
 Flow Cytometry | Flow-cytometry
using anti-human B2M antibody.HeLa cells were stained with an
irrelevant antibody (Blue Histogram) or an anti-human B2M antibody
monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a
concentration of 5 µg/ml for 30 mins at RT. After washing, bound
antibody was detected using a FITC conjugated goat anti-mouse antibody
(Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry | Flow-cytometry
using anti-human B2M antibody.Jurkat cells were stained with an
irrelevant antibody (Blue Histogram) or an anti-human B2M antibody
monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a
concentration of 5 µg/ml for 30 mins at RT. After washing, bound
antibody was detected using a FITC conjugated goat anti-mouse antibody
(Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry | Flow-cytometry
using anti-human B2M antibody.A431 cells were stained with an
irrelevant antibody (Blue Histogram) or an anti-human B2M antibody
monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a
concentration of 5 µg/ml for 30 mins at RT. After washing, bound
antibody was detected using a FITC conjugated goat anti-mouse antibody
(Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry | Flow-cytometry
using anti-human B2M antibody.Human peripheral blood lymphocytes were
stained with an irrelevant antibody (Blue Histogram) or an anti-human
B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram)
at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound
antibody was detected using a FITC conjugated goat anti-mouse antibody
(Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry | Flow-cytometry
using anti-human B2M antibody.Human peripheral blood lymphocytes were
stained with an irrelevant antibody (Blue Histogram) or an anti-human
B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram)
at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound
antibody was detected using a FITC conjugated goat anti-mouse antibody
(Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE | SDS-PAGE for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1). |
 Flow CytoMetry | Flow-cytometry
using APC anti-human B2M antibody. Hela cells were stained with an
irrelevant antibody (Blue Histogram) or an APC anti-human B2M monoclonal
antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5
µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte
Flow Cytometer. |
 Flow CytoMetry | Flow-cytometry
using FITC anti-human B2M antibody. Hela cells were stained with an
irrelevant antibody (Blue Histogram) or an FITC anti-human B2M
monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a
concentration of 5 µg/ml for 30 mins at RT. After washing, and cells
analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry | Flow-cytometry
using PE anti-human B2M antibody. Hela cells were stained with an
irrelevant antibody (Blue Histogram) or an PE anti-human B2M monoclonal
antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5
µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte
Flow Cytometer. |
 Western blot | Western blot analysis was performed using anti-CD1a & B2M monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD1a & B2M (Catalog No: HY043011) Lane 2: negative control |