产品概述
产品名称(Product Name) | Cleaved-TACE (R215) Rabbit Polyclonal Antibody |
描述(Description) | Rabbit Polyclonal Antibody |
宿主(Host) | Rabbit |
应用(Application) | WB,ELISA |
种属反应性(Reactivity) | Human,Rat,Mouse |
产品性能
偶联物(Conjugation) | Unconjugated |
修饰(Modification) | Unmodified |
同种型(Isotype) | IgG |
克隆(Clonality) | Polyclonal |
形式(Form) | Liquid |
存放说明(Storage) | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
储存溶液(Buffer) | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N. |
纯化方式(Purification) | Affinity purification |
免疫原
基因名(Gene Name) | ADAM17 |
别名(Alternative Names) | ADAM17; CSVP; TACE; Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD antigen CD156b |
基因ID(Gene ID) | 6868 |
蛋白ID(SwissProt ID) | P78536 |
产品应用
稀释比(Dilution Ratio) | WB 1:500-1:2000, ELISA 1:20000.Not yet tested in other applications. |
蛋白分子量(Molecular Weight) | 65kDa |
研究背景
ADAM metallopeptidase domain 17(ADAM17) Homo sapiens This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation ocatalytic activity:Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains.,cofactor:Binds 1 zinc ion per subunit.,domain:Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,function:Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.,induction:In arthritis-affected cartilage.,online information:Tumor necrosis factor alpha-converting enzyme entry,PTM:Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.,PTM:The precursor is cleaved by a furin endopeptidase.,similarity:Contains 1 disintegrin domain.,similarity:Contains 1 peptidase M12B domain.,subunit:Interacts with MAD2L1 and MUC1.,tissue specificity:Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.,
研究领域
Notch;Alzheimer's disease;Epithelial cell signaling in Helicobacter pylori infection;
关键字: ADAM17;Cleaved-TACE;(R215);Rabbit;Polyclonal;Antibody;一抗
武汉恩玑生命科技有限公司(EnkiLife)是一家深耕生命科学,专注细胞生物学和免疫学科研试剂的研发、生产与销售的生物技术企业,在全球100多个国家和地区开展业务,致力于为科研工作者提供高质量的产品和卓越的客户服务,推动生命科学的发展。
EnkiLife的产品线涵盖细胞系、原代细胞、细胞培养基、血清、细胞检测试剂盒、重组靶点蛋白、细胞因子、重组抗体、ELISA试剂盒、生化试剂盒等,并提供技术服务与定制开发,覆盖了生命科学研究的各大关键领域,包括细胞生物学、癌症、免疫学、神经科学、心血管疾病、干细胞、表观遗传学、内分泌、蛋白质组学、代谢组学等,全方位满足您的实验需求,让您享受科研的乐趣!
公司现已建立四大技术平台:
EnCyto?细胞培养及检测平台:拥有细胞系库(500+)、原代细胞库(500+)、基础培养基和完全培养基(1200+)
EnkiPro?重组蛋白平台:现货产品2000+,可提供定制化表达服务
EnAb?重组抗体平台:重组兔单抗(3000+),可提供定制化和标记服务
EnKits?试剂盒开发平台:可提供优质的ELISA试剂盒、配套试剂、抗体对、生化试剂盒等相关产品
EnkiLife在生产管理方面引入ISO9001质量管理体系和信息化、自动化的管理工具,拥有高效稳定的交付能力,与全球知名品牌建立了紧密的合作。
EnkiLife始终坚持以技术创新为驱动,以匠心铸就品质,以品质服务客户。
我们期待与更多的全球科研工作者和企业携手合作,共同推动生命科学领域的进步与发展。