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B-Raf 抗体

品牌：MedChemExpress (MCE)

货号：HY-P80036

存储条件：储存于 -20°C 下可保存 1 年。避免反复冻融。

运输条件：使用蓝冰运送。

产品活性：B-Raf Antibody 是一个非偶联、分子量约 84 kDa、小鼠来源的抗 B-Raf 单克隆抗体。它可用于人、小鼠、大鼠背景下 WB、IHC-P、ICC 实验，且不带标记。

宿主：鼠

反应物种：Human, Mouse, Rat

推荐稀释比例：WB: 1:500-1:2,000 ;ICC: 1:100-1:500 ;IHC-P: 1:100-1:500 Western blot analysis of extracts from K562(lane 2(20μg), HT-29(lane 3(20μg) and HEK293(lane 4(20μg) ) using B-Raf (HY-P80036) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80036, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

标签：Free

克隆性：Monoclonal

偶联：Non-conjugated

修饰：Unmodified

亚细胞定位：Nucleus, Cytoplasm, Cell membrane.

研究领域：Cell Biology

蛋白数据库：SwissProt: P15056 Human;SwissProt: P28028 Mouse

同型：IgG2b

敏感性：Endogenous

详情参考：www.medchemexpress.cn/antibody/b-raf-mouse-mab.html