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eIF4EBP1 抗体
品牌:MedChemExpress (MCE)
货号:HY-P80119
存储条件:储存于 -20°C 下可保存 1 年。避免反复冻融。
运输条件:使用蓝冰运送。
产品活性:eIF4EBP1 Antibody 是一个非偶联、分子量约 13 kDa、兔源、抗 eIF4EBP1 单克隆抗体。它可用于人、小鼠、大鼠背景下 WB、ICC/IF、IHC-P、IP、FC 实验,且不带标记。
宿主:兔
反应物种:Human, Mouse, Rat
推荐稀释比例:WB: 1:1,000-1:2,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 Western blot analysis of extracts from Hela (lane 1) and Raji (lane 2) and K562 (lane 3) using eIF4EBP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
标签:Free
克隆性:Recombinant, Monoclonal
偶联:Non-conjugated
修饰:Unmodified
亚细胞定位:Cytoplasm, cytosol.
研究领域:Epigenetics and Nuclear Signaling
蛋白数据库:SwissProt: Q13541 Human;SwissProt: Q60876 Mouse;SwissProt: Q62622 Rat
同型:IgG
敏感性:Endogenous
详情参考:www.medchemexpress.cn/antibody/eif4ebp1-rabbit-mab.html