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Phospho-AMPK alpha 1 抗体
品牌:MedChemExpress (MCE)
货号:HY-P80451
存储条件:储存于 -20°C 下可保存 1 年。避免反复冻融。
运输条件:使用蓝冰运送。
产品活性:Phospho-AMPK alpha 1 (Ser496) Antibody (YA226) 是一个非偶联、分子量约 64 kDa、兔源、抗磷酸化 AMPK alpha 1 (S496) 单克隆抗体。它可用于人背景下 WB、ICC/IF、IP 实验,且不带标记。
宿主:兔
反应物种:Human
推荐稀释比例:WB: 1:500-1:1,000 ;ICC/IF: 1:50-1:200 ;IP: Use at an assay dependent concentration. Western blot analysis of extracts from K562(lane 2(20μg) 、HUVEC (lane 3(20μg) and HEK293(lane 4(20μg)), using Phospho-AMPK alpha 1 (HY-P80451) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80451, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
标签:Free
克隆性:Recombinant, Monoclonal
偶联:Non-conjugated
修饰:Phosphorylated
亚细胞定位:Nucleus, Cytoplasm.
研究领域:Neuroscience
蛋白数据库:SwissProt: Q13131 Human
同型:IgG
敏感性:Endogenous
详情参考:www.medchemexpress.cn/antibody/phospho-ampk-alpha-1-s496-rabbit-mab.html