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Cytokeratin 19 抗体

品牌：MedChemExpress (MCE)

货号：HY-P81250

存储条件：储存于 -20°C 下可保存 1 年。避免反复冻融。

运输条件：使用蓝冰运送。

产品活性：Cytokeratin 19 Antibody 是一个非偶联、分子量约 44 kDa、鼠来源、抗 Cytokeratin 19 单克隆抗体。Cytokeratin 19 Antibody 可用于：人背景下 WB,IHC-F,IHC-P,ICC/IF 实验，且不带标记。

研究背景：结构蛋白（Structural Proteins） CK-19细胞角蛋白-19(又称CYFRa21-1)主要用于肿瘤方面的研究，近年来大多用于消化系统恶性肿瘤的鉴别，有学者认为：CK-19在胰腺干细胞分化过程中有细胞角蛋白19的表达，把细胞角蛋白19作为胰腺干细胞的标志物。阳性部位：主要在胞浆。

宿主：鼠

反应物种：Human

推荐稀释比例：WB: 1:500-2000; IHC-P: 1:100-500; IHC-F: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

基因 ID：3880

克隆性：Monoclonal

偶联：Non-conjugated

修饰：Unmodified

蛋白数据库：P08727

同型：IgG

同用名：40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd

敏感性：Endogenous

详情参考：www.medchemexpress.cn/antibody/cytokeratin-19-ya924.html