
Description
The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer. The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins. The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Features
Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
Three reference bands — 310 kDa (red), 75 kDa (red) and 25 kDa (green)
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, PM2800 3-color Extra Range Protein Marker resolves 13 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
关键字: 预染蛋白Marker;PM2800;Smobio代理;彩虹三色分子量:10-310KDa;10-310KDa;
【公司概况】北京鼎国生物成立于1997年,是国内成立最早的生物公司之一,公司拥有强大的营销团队,目前国内已有十七个分公司,在全国各地还有近三十多家长期业务合作伙伴。鼎国公司研发部设于北京市昌平区,面积2000多平方米,研发部拥有分子生物学、细胞生物学及免疫学实验所需大型仪器,能完成各类大型综合性实验服务。
【主营范围】鼎国生物的产品主要有试剂、试剂盒、耗材和仪器,涉及分子生物学、细胞生物学、免疫学、生物化学、微生物学、药物毒理学等。
【主营品牌】鼎国、Genview、德国Greiner耗材、Fisher耗材、Sigma-Vetec、SMBIO蛋白Marker、Scitecher胎牛血清、华美、Quawell分光光度计、东林仪器、博弗瑞德仪器等。
【企业理念】以人为本,追求个人与企业的共同发展。
【经营理念】诚信为本,质量至高,服务至上。
【管理理念】人文鼎国,文化鼎国,文明鼎国,建立高素质且有深厚凝聚力的团队。
广州鼎国生物技术有限公司成立于2001年,拥有一支专业、高效的销售和管理团队,经过十多年的发展,在广东市场获得良好的声誉。
【公司地址】广州市天河区东圃二马路61号东圃东湖工业区B栋2楼