重组羧肽酶B

重组羧肽酶B（EC 3.4.17.2）是一种金属依赖性外切蛋白酶，通过基因工程技术在大肠杆菌或酵母菌中表达并纯化，特异性水解多肽链C -末端的碱性氨基酸（赖氨酸Lys、精氨酸Arg、组氨酸His），是重组胰岛素及类似物生产、蛋白质测序与修饰、生物制药工艺优化的核心工具酶

Recombinant carboxypeptidase B (EC 3.4.17.2) is a metal‑dependent exopeptidase that is expressed and purified from E. coli or yeast using genetic engineering techniques. It specifically hydrolyzes basic amino acids (lysine, arginine, histidine) at the C‑terminus of polypeptide chains. It serves as a core tool enzyme in the production of recombinant insulin and its analogs, protein sequencing and modification, and biopharmaceutical process optimization.