更多产品描述请查看优宁维官网(https://www.univ-bio.com/vglu2/AGC-036-0.2ml.html?mk_channel=chemicalbook)
商品描述
Anti-VGLUT2 Antibody (#AGC-036) is a highly specific antibody directed against an epitope of rat Vesicular glutamate transporter 2. The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize VGLUT2 from rat, mouse and human samples.
背景
Central nervous system neurons have traditionally been thought to express exclusively membrane transporters and/or vesicular transporters for their transmitter. Three vesicular glutamate transporters (VGLUTs) have been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT3
1. These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission
2.
BNPI/DNPI encodes a membrane protein with 6-8 putative transmembrane domains which exhibits weak similarities to a kidney Na
+-dependent inorganic phosphate co-transporter
3. The transporters use a membrane potential gradient set by the vesicular H
+-ATPase for glutamate uptake
4. VGLUT1-3 are very similar in structure and function, but are used by different neuronal populations. VGLUT1 and VGLUT2 are expressed by the cortical and subcortical neurons respectively. VGLUT3 is expressed by non-glutamatergic neurons
5. VGLUT2 is expressed in the thalamus, brainstem, and deep cerebellar nuclei
2. A Recent study has shown that targeted deletion of VGLUT2 in mice causes perinatal lethality and a 95% reduction in evoked glutamatergic responses in thalamic neurons, although hippocampal synapses function normally. Behavioral analysis of heterozygous VGLUT2 mice showed discrete behavioral phenotypes that suggest a deficit in thalamic processing
6.