一、产品概述
Hu CD20 BV650 2H7 25Tst 是来自BD Pharmingen品牌的高品质产品，适用于科研实验领域。
更多产品描述请查看优宁维官网(https://www.univ-bio.com/ms4a1/563779.html?mk_channel=chemicalbook)
二、产品信息
| 货号 | 563780 |
| SKU货号 | 563779 |
| 品牌 | BD Pharmingen |
| 中文名称 | Hu CD20 BV650 2H7 25Tst |
| 英文名称 | Hu CD20 BV650 2H7 25Tst |
| 抗原名称 | CD20 |
| 宿主 | Mouse C57BL/6 IgG2b, κ |
| 免疫原 | Human 6.16c1.3 B cell line |
| 同种型 | Mouse C57BL/6 IgG2b, κ |
| 克隆号 | 克隆 2H7 (RUO) |
| 荧光素标记 | BV650 |
简单描述
The 2H7 monoclonal antibody specifically binds to CD20, encoded by the
MS4A1
(Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37 kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
商品描述
2H7
The 2H7 monoclonal antibody specifically binds to CD20, encoded by the
MS4A1
(Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37 kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
产品详情
BV650
The BD Horizon Brilliant Violet™ 650 (BV650) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 406-nm and an acceptor dye with an emission maximum (Em Max) at 649-nm. BV650, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 650-nm (e.g., a 660/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
格式:BV650
最多激发源:406 nm
最多发射源:649 nm
三、背景
| 别名 | MS4A1; B1; Bp35; LEU-16; S7 |
四、制备和贮存
| 保存方式 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
五、应用
| 反应种属 | Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development) |
| 实验应用 | Flow cytometry (Routinely Tested) |
| 目标/特异性 | CD20 |
| 推荐用量 | 5 µl |
六、产品特点
- 品质保证：经过严格的质量控制，确保产品的高品质和稳定性。
七、注意事项
- 本产品仅供科学研究使用，不可用于临床诊断或治疗。
- 请在通风良好的环境下操作，佩戴适当的个人防护装备。
- 如不慎接触皮肤或眼睛，请立即用大量清水冲洗并就医。