cDNA Synthesis Kit

cDNA Synthesis Kit / cDNA合成试剂盒11117831001 ，1 kit for up to 10 reactionsOptimized one-tube procedure for the synthesis of double-stranded cDNA from total RNA or mRNA.The ds cDNA can be used for the construction of non-directional cDNA libraries, as a starting point for substractive hybridization experiments to enrich differentially expressed genes, and for the in vitro transcription of whole cDNA populations to generate labeled cRNA for hybridization on DNA microarrays. Product DescriptionSample: 1 - 20 μg total RNA, 0.3 - 2 μg mRNAAssay time: 60 min for 1st strand synthesis, 120 min for 2nd strand synthesisProduct: double-stranded cDNA copy of an RNA sampleBackground InformationThe cDNA Synthesis System uses an optimized one-tube procedure, based on the method of Gubler and Hoffmann.