Recombinant Human G-CSF

Name

Recombinant Human G-CSF (Recombinant Human Granulocyte Colony Stimulation Factor; rHuG-CSF)；重组人粒细胞集落刺激因子

Synonyms

CSF3; C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3OS; filgrastim; Filgrastim; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin; Pluripoietin

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5% trehalose, pH4.0.

Endotoxin

Less than 1EU/μg of rHuG-CSF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition it may function in some adhesion or recognition events at the cell surface. 
In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (a.a.) precursor proteins, have been isolated. Both proteins have a 30a.a. signal peptide and have identical amino acid sequences except for a threea.a. insertion (deletion) at the 35tha.a. residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

Amino Acid Sequence

TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

产品编号

产品名称

包装

KL-5282-2μg

Recombinant Human G-CSF

2μg

KL-5282-10μg

Recombinant Human G-CSF

10μg

KL-5282-100μg

Recombinant Human G-CSF

100μg

KL-5282-1mg

Recombinant Human G-CSF

1mg

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说明书

1份