Recombinant Rat IFN-γ

Name

Recombinant Rat IFN-γ (Recombinant Rat Interferon-γ; rRtIFN-γ)；重组大鼠干扰素γ

Synonyms

IFNG; IFG; IFI; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1ng/ml, corresponding to a specific activity of >1.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH7.4, 1mM DTT, 5% Trehalose and 0.05% Tween-80.

Endotoxin

Less than 0.1EU/μg of rRtIFN-γ as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by Tlymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with murine IFN-γ (86%). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.

Amino Acid Sequence

QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC

产品编号

产品名称

包装

KL-6398-20μg

Recombinant Rat IFN-γ

20μg

KL-6398-100μg

Recombinant Rat IFN-γ

100μg

KL-6398-1mg

Recombinant Rat IFN-γ

1mg

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说明书

1份