Recombinant Murine Lungkine/CXCL15

Name

Recombinant Murine Lungkine/CXCL15 (Recombinant Murine Lungkine/CXCL15; rMuLungkine/CXCL15)；重组小鼠Lungkine/CXCL15

Synonyms

Il8; weche; Scyb15; lungkine

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 20-100ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µM filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20.

Endotoxin

Less than 1EU/µg of rMuLungkine/CXCL15 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Chemokines

Background

Mouse Lungkine/CXCL15, also named WECHE, is a member of the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein of 166 amino acids (aa) with a 25 aa predicted signal peptide and a 141 aa mature protein with an extremely long C terminal tail that protrudes beyond the chemokine fold. Mouse Lungkine shares 35% aa sequence identity with human ENA-78 and 31% identity with human IL-8. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the adult and fetal lung, and its expression is up-regulated under inflammatory conditions. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Studies from Lungkine knock out mice suggests that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace. Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation.

Amino Acid Sequence

QELRCLCIQE HSEFIPLKLI KNIMVIFETI YCNRKEVIAV PKNGSMICLD PDAPWVKATV GPITNRFLPE DLKQKEFPPA MKLLYSVEHE KPLYLSFGRP ENKRIFPFPI RETSRHFADL AHNSDRNFLR DSSEVSLTGS DA

产品编号

产品名称

包装

KL-6760-5µg

Recombinant Murine Lungkine/CXCL15

5µg

KL-6760-20µg

Recombinant Murine Lungkine/CXCL15

20µg

KL-6760-100µg

Recombinant Murine Lungkine/CXCL15

100µg

KL-6760-1mg

Recombinant Murine Lungkine/CXCL15

1mg

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说明书

1份