Recombinant Rat MIF

Name

Recombinant Rat MIF (Recombinant Rat Migration Inhibitor Factor; rRtMIF)；重组大鼠移动抑制因子

Synonyms

EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MMIF; Phenylpyruvate tautomerase

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Test in process.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, pH8.0, 150mM NaCl, 3% trehalose.

Endotoxin

Less than 1EU/μg of rRtMIF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Macrophage migration inhibitory factor (MIF ormMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99%, 90%, 89%, and 89%a.a. identical to human, murine, porcine and bovine, respectively.

Amino Acid Sequence

MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA

产品编号

产品名称

包装

KL7365-10μg

Recombinant Rat MIF

10μg

KL7365-50μg

Recombinant Rat MIF

50μg

KL7365-100μg

Recombinant Rat MIF

100μg

KL7365-1mg

Recombinant Rat MIF

1mg

-

说明书

1份