The production process of acridine:
Made from acridone. Acridone is obtained by cyclizing diphenylamine-2-carboxylic acid with sulfuric acid, and then reducing it to 9,10-dioxane with pentanol and sodium to oxidize to obtain acridine. Laboratory preparation can also be carried out by mixing acridone with zinc powder. The acridine sublimate formed by the reaction is dissolved in hydrochloric acid, and then alkalized with a sodium hydroxide solution to precipitate a precipitate, which is filtered to obtain a crude product. Recrystallization from methanol gave a fine melting point of 110 °C.

The physical properties of acridine:
Colorless needle crystal. The density is 1.005. The boiling point of 346 ° C, the melting point of 111 ° C (sublimation). Very slightly soluble in hot water, soluble in ethanol, ether and carbon disulfide. Both vapor and solution are irritating. The dilute solution is blue fluorescent.

The use of acridine:
Used in the manufacture of acridine dyes, acridine dyes are effective mutagens for phage, and the premise of a mutation induced by a phase-shift mutation appears to be a break in one strand of double-stranded DNA, such as in DNA replication or A break that occurred during the reorganization period. The mechanism of acridine-induced mutations is unclear and is generally presumed to be the result of insertion of a nucleic acid base pair with a dye molecule. Also used as a fluorescent pH indicator (fluorescence changes from green to blue when the pH is about 4.5-5.5). Acridine is an important parent compound for the manufacture of dyes and drugs such as acriflavine and quetiapine.

Synthesis and application of acridine derivatives:
Due to the special structure and functional unit of acridine and its important applications in many fields, the study of acridine has become a hot spot at home and abroad. The large conjugated ring has the functions of labeling and imaging in fluorescence, and can be embedded as Compounds in the DNA chain have been widely used in medicine and other fields, and the color price of the natural product is very similar in terms of dyes. In addition, as a photovoltaic material compound, it also shows a good potential value in replacing the metal semiconductor material in the organic semiconductor material. In recent years, new research and development fields such as microwave-assisted, metal-free catalysis, one-pot synthesis and other green, simple synthesis methods and organic semiconductor materials have emerged, which have brought more attention to the synthesis and application of acridine.