吡啰红Y
中文名称 | 吡啰红Y |
---|---|
中文同义词 | 派洛宁YPYRONI;PYRONING(Y) 吡啰红G 派洛宁G (Y);派洛宁Y, 高纯生物染料;派洛宁Y92-32-0;派洛宁Y(IND GRADE);吡罗红Y;派洛宁G;3,6-双(二甲基氨基)吨氯化物 |
英文名称 | PYRONIN Y |
英文同义词 | Pyronin Y,Pyronin G, PyroninJ, PyroninY;Pyronin G, C.I. 45005, for microscopy;Pyronin G (Pyronin Y);Pyronin y(G);Pyronin G, PyroninJ;Pyronin G, PyroninJ, PyroninY;Pyronin Y (CI x 45005);Pyronin Y,pure,high purity biological stain |
CAS号 | 92-32-0 |
分子式 | C17H19ClN2O |
分子量 | 302.8 |
EINECS号 | 202-147-8 |
相关类别 | 血液学和组织学;生物化学;色素类;色素;染色剂和染料;生化试剂-色素类;化学试剂;生化试剂;通用生化试剂-生物染色剂与化学指示剂;Xanthene |
Mol文件 | 92-32-0.mol |
结构式 |
吡啰红Y 性质
熔点 | 250-260 °C(lit.) |
---|---|
储存条件 | room temp |
溶解度 | 易溶于水;微溶于乙醇、乙二醇、甲基溶纤剂 |
形态 | 粉末/固体 |
色指数 | 45005 |
颜色 | 粉色 |
水溶解性 | Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL). |
最大波长(λmax) | 548 nm |
Merck | 13,8097 |
BRN | 3795789 |
稳定性 | 稳定的。与强氧化剂不相容。 |
生物领域应用 | Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides |
InChIKey | INCIMLINXXICKS-UHFFFAOYSA-M |
CAS 数据库 | 92-32-0 |
EPA化学物质信息 | Pyronine Y (92-32-0) |
Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin.
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting.
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells.
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent.
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA).
安全信息
危险品标志 | Xn,Xi |
---|---|
危险类别码 | 68-36/37/38 |
安全说明 | 22-24/25-36-26 |
WGK Germany | 3 |
RTECS号 | BQ1450000 |
TSCA | Yes |
海关编码 | 32041300 |
提供商 | 语言 |
---|---|
英文
|
|
英文
|