金黄紫堇碱
| 中文名称 | 金黄紫堇碱 |
|---|---|
| 中文同义词 | 金黄紫堇碱;(S)-金黄紫堇碱;斯氏紫堇碱;(S)-3,10-二甲氧基-5,8,13,13A-四氢-6H-异喹啉并[3,2-A]异喹啉-2,9-二醇 |
| 英文名称 | SCOULERIN |
| 英文同义词 | SCOULERIN;SCOULERIN(SH);(13aS)-5,8,13,13a-tTtrahydro-3,10-diMethoxy-6H-dibenzo[a,g]quinolizine-2,9-diol;(S)-5,8,13,13a-Tetrahydro-3,10-diMethoxy-6H-Dibenzo[a,g]quinolizine-2,9-diol;Alkaloid HF 1, froM HunneMannia fuMariaefolia;(-)-scoulerine;3,10-dimethoxy-13a-alpha-berbine-2,9-diol;3,10-dimethoxy-13a-alpha-berbine-9-diol |
| CAS号 | 6451-73-6 |
| 分子式 | C19H21NO4 |
| 分子量 | 327.37 |
| EINECS号 | 200-258-5 |
| 相关类别 | 植物提取物;标准品;生物碱;对照品;Aromatics;Heterocycles;Intermediates & Fine Chemicals;Alkaloids;Pharmaceuticals |
| Mol文件 | 6451-73-6.mol |
| 结构式 |  |
金黄紫堇碱 性质
| 熔点 | 192°C |
|---|---|
| 沸点 | 503.3±50.0 °C(Predicted) |
| 密度 | 1.37±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | 溶于二甲基亚砜 |
| 形态 | 结晶固体 |
| 酸度系数(pKa) | 9.88±0.20(Predicted) |
Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC
50
s ranging from 2.7 µM to 6.5 µM.
Scoulerine (2.5-20 µM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells.
Scoulerine (2.5-20 µM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis.
Scoulerine induces G2 or M cell cycle arrest.
Scoulerine (2.5-5 µM; 24 hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells.
Scoulerine (2.5-5 µM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner.
Scoulerine (5-10 µM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells
Cell Proliferation Assay
| Cell Line: | Jurkat and MOLT-4 cells |
| Concentration: | 2.5, 5, 10, 15 and 20 µM |
| Incubation Time: | 24 hours |
| Result: | Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner. |
Apoptosis Analysis
| Cell Line: | MOLT-4 and Jurkat cells |
| Concentration: | 2.5, 5, 10, 15 and 20 µM |
| Incubation Time: | 24 hours |
| Result: | Induced MOLT-4 and Jurkat cells apoptosis. |
Cell Cycle Analysis
| Cell Line: | Jurkat and MOLT-4 leukemic cells |
| Concentration: | 2.5-20 µM |
| Incubation Time: | 16 hours |
| Result: | Induced cell cycle arrest at the G2/M transition. |
Western Blot Analysis
| Cell Line: | MOLT-4 cells |
| Concentration: | 2.5, 5 µM |
| Incubation Time: | 24 hours |
| Result: | Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells. |