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Bromelain from pineapple stem 2 u/mg C화학적 특성, 용도, 생산
개요
Bromelain is a major sulfhydryl proteolytic enzyme found in pineapple plants (Ananas comosus (L.) Merryl). Bromelain is usually found in the fruit and stem, but it is also easy to find in small amounts in the leaf, skin, core, etc. Bromelain from pineapple stem is known as stem bromelain (SBM). SBM has a unique content compared with bromelain from fruit (FBM) because SBM contains many mixtures of various types of thiol-endopeptidases. Based on the research of Heinecke and Gortner, SBM concentrations are better than FBM. Bromelain has been reported to have medical efficacy. Bromelain has been used traditionally to relieve tense muscles, inflammation, and connective tissue. In recent years, bromelain has been used as an anti-inflammatory, antiedematous, and antithrombotic agent (preventing blood clotting). In addition, bromelain showed activity in preventing lung metastases and potentially as an anticancer agent[1].
화학적 성질
Yellow-brown powder
용도
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
Purification Methods
This protease has been purified via the acetone powder, G-75 Sephadex gel filtration and Bio-Rex 70 ion-exchange chromatography, and has 1cm 20.1 at 280nm. The protease from pineapple hydrolyses benzoyl glycine ethyl ester with a Km (app) of 210mM and kcat of 0.36 sec-1 . [Murachi Methods Enzymol 19 273 1970, Balls et al. Ind Eng Chem 33 950 1941.]
