페니실린 감마-일라디산염 세포 C화학적 특성, 용도, 생산
화학적 성질
White powder
역사
Penicillin was discovered by chance in 1928 by Alexander Fleming. He
observed that the growth of a bacteria culture was inhibited by a fungus
Penicillum notatum. He published his results but did not pursue its
industrial development actively. Ten years later, H. Florey and coworkers
had produced enough purified penicillin to treat just one patient.
This test, however, was sufficient to prove that it was a viable drug.
From then on many people and companies participated in the development
of new fermentation technologies, new microorganisms, new downstream
processing, and so on to make a large-scale production possible.
Penicillin did not only change the medical world, but also the fermentation
technology. The naturally growing (wild type) Penicillum notatum
produced penicillin with a yield of 10 mg/L. Therefore, the first task was
the search for a more productive species. Eventually, Penicillium chrysogenum
was identified as the most productive species. To enhance penicillin
production further, the old method of growing Penicillum mold on
the surface of the medium in liter-sized flasks was replaced by fermentation
in large aerated tanks. This allowed the mold to grow throughout
the entire tank and not just on the surface of the medium. Today,
penicillin and other antibiotics are produced in large-scale fermenters
holding several hundred cubic meters of medium and the yield has
increased 5000 fold to 50 g/L.
용도
Penicillin G sodium salt is used as a β-lactam antibiotic. Penicillin G can be used as a selective agent in several types of isolation media. It has been used to study the diagnostic and therapeutic implications of gentamicin-resistant Enterococcus faecalis sequence type 6 with reduced penicillin susceptibility and in cell culture both alone and combined with streptomycin and other antibiotics.
정의
penicillin: An antibiotic derivedfrom the mould Penicillium notatum;specifically it is known as penicillin Gand belongs to a class of similar substancescalled penicillins. They producetheir effects by disruptingsynthesis of the bacterial cell wall,and are used to treat a variety of infectionscaused by bacteria.
World Health Organization (WHO)
Penicillin is listed separately in the WHO
Model List of Essential Drugs.
일반 설명
White to slightly yellow crystalline powder with a faint odor. pH (10% solution) 5.5-7.5.
공기와 물의 반응
Water soluble.
반응 프로필
Penicillin G sodium salt is hygroscopic. Penicillin G sodium salt is incompatible with acids, oxidizing agents (especially in the presence of trace metals), heavy metal ions such as copper, lead, zinc and mercury; glycerol, sympathomimetic amines, thiomersal, wood alcohols, cetostearyl alcohol, hard paraffins, macrogols, cocoa butter and many ionic an nonionic surface-active agents. Penicillin G sodium salt is also incompatible with alkalis, compounds leached from vulcanized rubber, hydrochlorides of tetracyclines and organic peroxides. Other incompatibilities include reducing agents, alcohols, other hydroxy compounds, self-emulsifying stearyl alcohol, emulsifying wax, lanolin, crude cholinesterated bases, glycol, sugars, amines, aminacrine hydrochloride, ephedrine, procaine, rubber tubing, thiamine hydrochloride, zinc oxide, oxidized cellulose, iodine, iodides, thiols, chlorocresol and resorcinol. Penicillin G sodium salt may also be incompatible with naphthalene oils and vitamin B.
화재위험
Flash point data for Penicillin G sodium salt are not available; however, Penicillin G sodium salt is probably combustible.
Safety Profile
Poison by intracerebral,parenteral, and intramuscular routes. Moderately toxic viaintravenous route. Mildly toxic by ingestion. Experimentalteratogenic and reproductive effects. Questionablecarcinogen with experimental tumorigenic data. Whenheated to
Purification Methods
Purify the salt by dissolving it in a small volume of MeOH (in which it is more soluble than EtOH) and treating gradually with ~5 volumes of EtOAc. This gives an almost colourless crystalline solid (rosettes of clear-cut needles) and recrystallising twice more if slightly yellow in colour. The salt has also been conveniently recrystallised from the minimum volume of 90% Me2CO and adding an excess of absolute Me2CO. A similar procedure can be used with wet n-BuOH. If yellow in colour, then dissolve (~3.8g) in the minimum volume of H2O (3mL), add n-BuOH and filter through a bed of charcoal. The salt forms long white needles on standing in a refrigerator overnight. More crystals can be obtained on concentrating the mother liquors in vacuo at 40o. A further recrystallisation (without charcoal) yields practically pure salt. A good preparation has ~600 Units/mg. The presence of H2O in the solvents increases the solubility considerably. The solubility in mg/100mL at 0o is 6.0 (Me2CO), 15.0 (Me2CO/0.5% H2O), 31.0 (Me2CO/1.0% H2O), 2.4 (methyl ethyl ketone), 81.0 (n-butanol) and 15.0 (dioxane at 14o). Alternatively it is dissolved in H2O (solubility is ~10%), filtered if necessary and precipitated by addition of EtOH and dried in a vacuum over P2O5. A sample can be kept for 24hours at 100o without loss of physiological activity. It also crystallises from MeOH/EtOAc. [IR: Barnes et al. Anal Chem 19 620 1947, The Chemistry of Penicillin (Clarke, Johnson and Robinson eds.) Princeton University Press, Princeton NJ, Chapter V 85 1949, Beilstein 27 III/IV 5861.]
페니실린 감마-일라디산염 세포 준비 용품 및 원자재
원자재
준비 용품