| | 27072-45-3 Basic information More.. |
| Product Name: | Fluorescein isothiocyanate | | Synonyms: | FluoresceinIsothiocyanate,Isomers1&11;Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-5(or 6)-isothiocyanato-;Fluorescein 5(6)-isothiocyanate,FITC, Fluorescein isothiocyanate;Fluorescein5(6)-isot;5(6)-FITC;Fluorescein 5(6)-isothiocyanat, mixture of isomers >90%;Fluorescein Isothiocyanate (Mixture of 5- and 6- isoMers);Isothiocyanatofluorescein | | CAS: | 27072-45-3 | | MF: | C21H11NO5S | | MW: | 389.38 | | EINECS: | 248-207-7 | | Mol File: | 27072-45-3.mol |  |
Use
Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0
|
|
27072-45-3
Recommend Suppliers |
|