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| | SUC-LEU-LEU-VAL-TYR-AMC Basic information |
| Product Name: | SUC-LEU-LEU-VAL-TYR-AMC | | Synonyms: | Z-LLVY-AMC;succinyl-leucyl-leucyl-valyl-tyrosyl-methylcoumarinamide;SUC-LEU-LEU-VAL-TYR-AMC;SUC-LEU-LEU-VAL-TYR-MCA;SUC-LEU-LEU-VAL-TYR-7-AMINO-4-METHYLCOUMARIN;SUCCINYL-L-LEUCYL-L-LEUCYL-L-VALYL-L-TYROSINE-4-METHYLCOUMARINYL-7-AMIDE;SUCCINYL-L-LEUCYL-L-LEUCYL-L-VALYL-L-TYROSINE 4-METHYLCOUMARYL-7-AMIDE;PROTEASOME 20S, (SUC-LEU-LEU-VAL-TYR-AMC) | | CAS: | 94367-21-2 | | MF: | C40H53N5O10 | | MW: | 763.88 | | EINECS: | | | Product Categories: | Pepetides | | Mol File: | 94367-21-2.mol |  |
| | SUC-LEU-LEU-VAL-TYR-AMC Chemical Properties |
| Boiling point | 1116.8±65.0 °C(Predicted) | | density | 1.249±0.06 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | Soluble in DMSO (up to 20 mg/ml), or in DMF (up to 10 mg/ml) | | form | Lyophilized solid | | pka | 4.69±0.10(Predicted) | | color | White to Off-White | | Sequence | Suc-Leu-Leu-Val-Tyr-AMC | | Stability: | Stable for 1 year from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20°C for up to 1 month. |
| | SUC-LEU-LEU-VAL-TYR-AMC Usage And Synthesis |
| Description | Suc-Leu-Leu-Val-Tyr-AMC (94367-21-2) is a fluorogenic substrate for the chymotryptic activity of the 20S proteasome1 and other chymotrypsin-like proteases, as well as calpains2. A commonly used substrate for assaying proteasomal enzymatic activity.3,4 Excitation max.: 360 nm; emission max.: 460 nm. | | Uses | Suc-Leu-Leu-Val-Tyr-AMC is a fluorgenic substrate for chymotrypsin-like proteases, as well as calpains. It can be used to treat tuberculosis. | | Definition | ChEBI: A tetrapeptide compound with a succinyl group at the N-terminal and a 7-amino-4-methylcoumarin group at the C-terminal. | | Biochem/physiol Actions | In the presence of chymotrypsin-like enzyme activity, the fluorophore, 7-amido-4-methylcoumarin is released from N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin. The fluorescence obtained is a measure of the enzyme activity.6 | | References | 1) Stein et al. (1996), Kinetic characterization of the chymotryptic activity of the 20S proteasome; Biochemistry, 35 3899
2) Sasaki et al. (1984), Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates; J. Biol. Chem., 259 12489
3) Hamouda et al. (2014), The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells; Oncotarget, 5 6252
4) Min et al. (2017), USP14 inhibitor attenuates cerebral ischemia/reperfusion-induced neuronal injury in mice; J. Neurochem,, 140 826 |
| | SUC-LEU-LEU-VAL-TYR-AMC Preparation Products And Raw materials |
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