69-78-3
69-78-3 结构式
基本信息
5,5’-二硫双(2-硝基苯甲酸)
二硫代硝基苯甲酸
5,5'-二硫代-双(硝基苯甲酸)
5,5＇二硫代双(2-硝基苯甲酸)
6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸
双(3-羧基-4-硝基苯基)二硫
5,5'-二硫代二(2-硝基苯甲酸)
5,5'-二巯基(2-硝基苯甲酸)
DTNB,5,5-二硫基-双(2-硝基苯甲酸)
5.5-硫代双(2-硝基苯甲酸)
3-CARBOXY-4-NITROPHENYL DISULFIDE
5-[(3-CARBOXY-4-NITROPHENYL)DITHIO]-2-NITROBENZOIC ACID
5,5'-DITHIOBIS(2-NITROBENZOIC ACID)
5,5-DITHIO-BIS(2-NITROBENZOIC ACID)
6,6'-DINITRO-3,3'-DITHIODIBENZOIC ACID
BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE
DTNB
ellmann's reagent
ELLMAN'S REAGENT
ELLMANS' REAGENT
2,2’-dinitro-5,5’-dithiodibenzoesaeure
3,3’-dithiobis(6-nitro-benzoicaci
3,3’-dithiobis(6-nitrobenzoicacid)
3,3’-dithiobis[6-nitro-benzoicaci
5,5’-dithiobis(2-nitrobenzoic
dithionitrobenzoicacid
Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans
Ellmanns
5,5'-Dithobis(2-Nitrobenzoic Acid)
物理化学性质
安全数据
常见问题列表
• 巯基测定 — 与游离（还原）巯基基团 (—SH) 定量反应，以生成可检测的 TNB 产物
• 比色检测 — 有色产物可在比色皿或微孔板中进行分光光度测量
• 成熟的分析方法 — 可利用消光系数计算或通过与半胱氨酸标准进行比较，对肽或蛋白巯基基团进行定量测定
DTNB reacts with the free sulfhydryl side chain of cysteine to form an S-S bond between the protein and a thionitrobenzoic acid (TNB) residue.
The main advantage of DTNB over alternative reagents (e.g., N-ethylmaleimide or iodoacetamide) is in the selectivity of this reagent and in the ability to follow the course of the reaction spectrophotometrically.
Modification of Enzyme with DTNB:
Modification of the SH groups of the enzyme is carried out by reacting 10 μL of 10 mM DTNB solution (about a 20-fold molar excess) at room temperature with 0.6 mL of enzyme solution (0.807 mg/mL) in 80 mM phosphate buffer, pH 8.0, containing 1 mM EDTA, which has been dialyzed previously against the same buffer solution for 24 h. The number of SH groups is estimated from the increase of absorbance at 410 nm using a molar extinction coefficient of 13,600 M
-1
cm
-1
for thionitrobenzoate ions liberated.
| 报价日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
| 2024/04/30 | A14331 | 5,5'-二硫双(2-硝基苯甲酸), 99% 5,5'-Dithiobis(2-nitrobenzoic acid), 99% | 69-78-3 | 1g | 351元 |
| 2024/04/30 | C11754 | 5,5'-二硫双(2-硝基苯甲酸) 5,5'-Dithiobis-(2-nitrobenzoic acid), 99% | 69-78-3 | 1g | 683元 |
| 2024/04/30 | C11754 | 5,5'-二硫双(2-硝基苯甲酸) 5,5'-Dithiobis-(2-nitrobenzoic acid), 99% | 69-78-3 | 5g | 910元 |